Composite

Part:BBa_K3052059:Design

Designed by: Xiaoyun Lu   Group: iGEM19_XJTU-CHINA   (2019-10-16)


CI+Pλ+DHJ+ho1-pcyA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2739
    Illegal NheI site found at 2762
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4299
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1519
    Illegal PstI site found at 1600
    Illegal PstI site found at 2023
    Illegal PstI site found at 2089
    Illegal PstI site found at 2179
    Illegal NgoMIV site found at 1050
    Illegal NgoMIV site found at 3852
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains a SapI site in order to perform Golden Gate Assembly with another part.


Source

This part was assembled by different parts through Golden Gate Assembly.

References